Thursday, 25 October 2012


Characterization of Biofilm Formation by Borrelia burgdorferi In Vitro

published 24 October 2012 Plos One here 

Abstract Top

Borrelia burgdorferi, the causative agent of Lyme disease, has long been known to be capable of forming aggregates and colonies. It was recently demonstrated that Borrelia burgdorferi aggregate formation dramatically changes the in vitro response to hostile environments by this pathogen. In this study, we investigated the hypothesis that these aggregates are indeed biofilms, structures whose resistance to unfavorable conditions are well documented. We studied Borrelia burgdorferi for several known hallmark features of biofilm, including structural rearrangements in the aggregates, variations in development on various substrate matrices and secretion of a protective extracellular polymeric substance (EPS) matrix using several modes of microscopic, cell and molecular biology techniques. The atomic force microscopic results provided evidence that multilevel rearrangements take place at different stages of aggregate development, producing a complex, continuously rearranging structure. Our results also demonstrated that Borrelia burgdorferi is capable of developing aggregates on different abiotic and biotic substrates, and is also capable of forming floating aggregates. Analyzing the extracellular substance of the aggregates for potential exopolysaccharides revealed the existence of both sulfated and non-sulfated/carboxylated substrates, predominately composed of an alginate with calcium and extracellular DNA present. In summary, we have found substantial evidence that Borrelia burgdorferi is capable of forming biofilm in vitro. Biofilm formation by Borrelia species might play an important role in their survival in diverse environmental conditions by providing refuge to individual cells.

Dr Eva Sapi link to Utube presentation on Biofilms here

Dr Alan B  MacDonald 'Six years ago,a a Symposium at the University of New Haven I delivered a lecture on biofilms of Borrelia burgdorferi (Bb). The PDF File of the PPT and its Video have been posted on the Web and are freely downloadable.' here

Dr Alan B MacDonald's website here
Dr Alan B MacDonald Alzheimer's Disease here 

Earlier posts on Dr Alan B MacDonald here

Dr Alan B MacDonald will present at 2012 International Lyme and Associated Diseases conference details here

1 comment:

  1. ANYONE THat was told they have Fibromyalgia would be tested thouroughy for chronic or late stage Lyme disease. If the standard tests come back negative you should a direct blood Culture down through advanced labrotories on in the Northeast of the US. This id the most aaccurate test there is out there.