Lyme Disease and Dementia, Lecture and Power Point presentation by Dr Alan MacDonald from John E Grinstein on Vimeo.
What causes Alzheimer's Disease or Dementia?
Such an important question - The Alzheimer's Society estimates that there will soon be 850,000 people in Britain living with dementia, the majority of whom are wholly or partly due to Alzheimer's disease. The economic costs of this will rise to over £26 billion.
The above Vimeo lecture from Dr Alan MacDonald details his work and modern technological developments that are able to show Borrelia infection in Alzheimer's brains.
With latest technology Flouresence In Situ DNA Hybridisation (FISH) and DNA Borrelia Burgdorferi specific probes he shows further studies he has done with peripheral blood https://cloud.gonitro.com/p/iaaxzXsfbUBRef55xbwmcM
and Spinal Fluid https://cloud.gonitro.com/p/c3E6l8iDP5Jl1c0dUcG3Bu
Dr MacDonald further says -
Molecular Beacon DNA probes for two open reading frames in the Borrelia burgdorferi chromosome have been developed and Validated for their specificity in hybridizing uniquely to burgdorferi Borrelia DNA.
RESULTS OF VALIDATION STUDIES:
Borrelia burgdorferi pure culture strain ATCC 35210 = POSITIVE
Borrelia Hermsii ,pure culture = NEGATIVE
Also Negative results with ALL. OF. THE FOLLOWING. : Normal human blood, normal by cal squamous epithelial cells,
oral bacterial microbes, E.coli, Candida albicans
Cerebrospinal fluid submitted from a volunteer for molecular evaluation for possible Borrelia DNA IN THE SPINAL FLUID.
( Hospital testing of the spinal fluid produced Normal glucose, Normal protein, normal white blood cell and red blood cell counts,No bacteria by culture of spinal fluid, no spirochetes seen in cytology examination
MolecularBeacon DNA PROBE RESULTS : Positive for Borrelia burgdorferi spirochetes
Conclusions:
KENTUCKY resident, adult male, chronically ill, exact cause of chronic illness not diagnosed after multiple physician visits
Lyme ELISA : NEGATIVE
Lyme WESTERN BLOT : NEGATIVE ( only 41 KD band present)
Patient gives a history of multiple tick bites in Kentucky, no erythema Migrans rash noted at any time
Summation: DNA PROBES (2) demonstrate. Many Borrelia burgdorferi in the cerebrospinal fluid by
FISH method ( Fluorescence In Situ Hybridization) . Many spirochetes seen in fresh up concentrated spinal fluid
By FISH, indicating a very heavy load of infection.
No host immune or leukocytic , macrophage response to the spinal fluid BorrelIa burgdorferispirochetes.
Chronic illness - un-categorized with conventional medical testing -
Diagnosis: Chronic Lyme Borreliosis, co- infection status not yet addressed by additional proper testing
and from Dr MacDonald -
The FISH DNA hybridization. Will settle the heretofore contentious debates about the REALITY OF CHRONIC BORRELIA ILLNESSES.
I WILL PROVIDE THE METHODOLOGY AND THE DETAILS OF MANUFACTURE OF THE
MOLECULAR beacon DNA PROBES IN AN UPCOMING PEER REVIEWED PUBLICATION.
AN Epifluorescent microscope is required to accomplish the microscopic exams.
For a limited time I will continue to receive blood smears and Spinal fluid specimens
To extend these observations.
I will donate the peer reviewed methodology Free of charge to persons who
Own an Epifluorescent microscope.
The Inconvenient Truth of the reality of Seronegative Lyme Borreliosis or IgM reactive serology in Patients with " Late " chronic Borreliosis. Will be embraced as multiple scientists in international Laboratories. Implement the FISH METHOD. for Borrelia detection in blood, seminal fluid, spinal fluid,amniotic fluid,
Arteritis
Or...chamber or the eye Aqueous ...
and biopsies from solid organs or examination of autopsy tissues, with the FISH METHOD USINGBORRELIA BURGDORFERI
DNA hybridization will settle the contentious category of chronic Borreliosis,
Once and for all time...
Links to additional FISH POSITIVE RESULTS FROM CHRONIC BORRELIOSIS AND FROM AUTOPSY CHRONIC BORRELIOSIS:
http://www.lymeneteurope.org/forum/viewtopic.php?f=7&t=5681
Further information and images -
http://www.lymeneteurope.org/forum/viewtopic.php?f=5&t=5664&p=41363#p41450
http://www.lymeneteurope.org/forum/viewtopic.php?f=6&t=5695
http://f1000.com/posters/browse/summary/1097535
and Spinal Fluid https://cloud.gonitro.com/p/c3E6l8iDP5Jl1c0dUcG3Bu
Dr MacDonald further says -
Molecular Beacon DNA probes for two open reading frames in the Borrelia burgdorferi chromosome have been developed and Validated for their specificity in hybridizing uniquely to burgdorferi Borrelia DNA.
RESULTS OF VALIDATION STUDIES:
Borrelia burgdorferi pure culture strain ATCC 35210 = POSITIVE
Borrelia Hermsii ,pure culture = NEGATIVE
Also Negative results with ALL. OF. THE FOLLOWING. : Normal human blood, normal by cal squamous epithelial cells,
oral bacterial microbes, E.coli, Candida albicans
Cerebrospinal fluid submitted from a volunteer for molecular evaluation for possible Borrelia DNA IN THE SPINAL FLUID.
( Hospital testing of the spinal fluid produced Normal glucose, Normal protein, normal white blood cell and red blood cell counts,No bacteria by culture of spinal fluid, no spirochetes seen in cytology examination
MolecularBeacon DNA PROBE RESULTS : Positive for Borrelia burgdorferi spirochetes
Conclusions:
KENTUCKY resident, adult male, chronically ill, exact cause of chronic illness not diagnosed after multiple physician visits
Lyme ELISA : NEGATIVE
Lyme WESTERN BLOT : NEGATIVE ( only 41 KD band present)
Patient gives a history of multiple tick bites in Kentucky, no erythema Migrans rash noted at any time
Summation: DNA PROBES (2) demonstrate. Many Borrelia burgdorferi in the cerebrospinal fluid by
FISH method ( Fluorescence In Situ Hybridization) . Many spirochetes seen in fresh up concentrated spinal fluid
By FISH, indicating a very heavy load of infection.
No host immune or leukocytic , macrophage response to the spinal fluid BorrelIa burgdorferispirochetes.
Chronic illness - un-categorized with conventional medical testing -
Diagnosis: Chronic Lyme Borreliosis, co- infection status not yet addressed by additional proper testing
and from Dr MacDonald -
The FISH DNA hybridization. Will settle the heretofore contentious debates about the REALITY OF CHRONIC BORRELIA ILLNESSES.
I WILL PROVIDE THE METHODOLOGY AND THE DETAILS OF MANUFACTURE OF THE
MOLECULAR beacon DNA PROBES IN AN UPCOMING PEER REVIEWED PUBLICATION.
AN Epifluorescent microscope is required to accomplish the microscopic exams.
For a limited time I will continue to receive blood smears and Spinal fluid specimens
To extend these observations.
I will donate the peer reviewed methodology Free of charge to persons who
Own an Epifluorescent microscope.
The Inconvenient Truth of the reality of Seronegative Lyme Borreliosis or IgM reactive serology in Patients with " Late " chronic Borreliosis. Will be embraced as multiple scientists in international Laboratories. Implement the FISH METHOD. for Borrelia detection in blood, seminal fluid, spinal fluid,amniotic fluid,
Arteritis
Or...chamber or the eye Aqueous ...
and biopsies from solid organs or examination of autopsy tissues, with the FISH METHOD USINGBORRELIA BURGDORFERI
DNA hybridization will settle the contentious category of chronic Borreliosis,
Once and for all time...
Links to additional FISH POSITIVE RESULTS FROM CHRONIC BORRELIOSIS AND FROM AUTOPSY CHRONIC BORRELIOSIS:
http://www.lymeneteurope.org/forum/viewtopic.php?f=7&t=5681
Further information and images -
http://www.lymeneteurope.org/forum/viewtopic.php?f=5&t=5664&p=41363#p41450
http://www.lymeneteurope.org/forum/viewtopic.php?f=6&t=5695
http://f1000.com/posters/browse/summary/1097535
For over 100 years it has been known that the spirochete Treponema pallidum, the causative agent of Syphilis causes slowly progressive dementia, cortical atrophy and amyloid deposition (1)
It is recognised that spirochetal infection Borrelia (Lyme Disease) can also cause Alzheimer's and Dementia (1,2,3,4).
Sadly over reliance on unreliable antibody tests has held back the science in the field of diagnosing Borrelia infections. (5)
Sadly over reliance on unreliable antibody tests has held back the science in the field of diagnosing Borrelia infections. (5)
Prof Judith Miklossy (1) and the Lancashire University Dental department found Dental spirochetes in Alzheimer's brains. (3,6)
A question on my post on Twitter 'Page 2- What is PanDNA? The title says "not specific", then the caption says "unique specificity"
ReplyDeleteAnswer from Dr MacDonald
The PanDna stain is Ethidium bromide. It is an intercalating Fluorochrome which sits between the double strand DNA, and produces red to pink color upon UV LIGHT EXPOSURE.
THE FISH is a DNA HYBRIDIZING METHOD, ...QUITE SPECIFIC FOR BURGDORFERI.
IF A PERFECT DNA MATCH IS NOT PRESENT, THEN EACH OF THE TWO PROBES
147 and 740 will fail to bind to the spirochetes and no fluorescent signal will be visible.
If the spirochete density in Ethidium bromide staining exceeds the spirochete density in the FISH METHOD, the possibility of simultaneous double strain infection exists,
The First which fails the perfect match with the 2 burgdorferi DNA PROBES,
AND THE SECOND STRAIN WHICH PERFECTLY MATCHES BOTH DNA probes and produces a fluorescent HYBRIDIZATION SIGNAL ( blue color or grre color with appropriate barrier filters)
Hybridized DNA probes in my system NEVER PRODUCE A RED OR A PINK SIGNAL,,!
and further molecular work is necessary to identify the Ethidium bromide spirochetes which fail to,
HYBRIDIZE WITH THE TWO BORRELIA SPECIFIC DNA PROBES FOR Burgdorferi type Borrelia.