tag:blogger.com,1999:blog-3707770726760676731.post6524658384041064118..comments2024-02-25T19:33:19.408+00:00Comments on LOOKING AT LYME DISEASE - MSIDS: WHAT CAUSES ALZHEIMER'S DISEASE OR DEMENTIA?Joannehttp://www.blogger.com/profile/12905137222286141548noreply@blogger.comBlogger1125tag:blogger.com,1999:blog-3707770726760676731.post-60289535738437531012015-01-31T10:33:08.384+00:002015-01-31T10:33:08.384+00:00A question on my post on Twitter 'Page 2- What...A question on my post on Twitter 'Page 2- What is PanDNA? The title says "not specific", then the caption says "unique specificity"<br />Answer from Dr MacDonald<br />The PanDna stain is Ethidium bromide. It is an intercalating Fluorochrome which sits between the double strand DNA, and produces red to pink color upon UV LIGHT EXPOSURE.<br />THE FISH is a DNA HYBRIDIZING METHOD, ...QUITE SPECIFIC FOR BURGDORFERI.<br />IF A PERFECT DNA MATCH IS NOT PRESENT, THEN EACH OF THE TWO PROBES<br />147 and 740 will fail to bind to the spirochetes and no fluorescent signal will be visible.<br /><br /><br />If the spirochete density in Ethidium bromide staining exceeds the spirochete density in the FISH METHOD, the possibility of simultaneous double strain infection exists,<br />The First which fails the perfect match with the 2 burgdorferi DNA PROBES,<br />AND THE SECOND STRAIN WHICH PERFECTLY MATCHES BOTH DNA probes and produces a fluorescent HYBRIDIZATION SIGNAL ( blue color or grre color with appropriate barrier filters) <br />Hybridized DNA probes in my system NEVER PRODUCE A RED OR A PINK SIGNAL,,!<br /><br /> and further molecular work is necessary to identify the Ethidium bromide spirochetes which fail to,<br />HYBRIDIZE WITH THE TWO BORRELIA SPECIFIC DNA PROBES FOR Burgdorferi type Borrelia.Joannehttps://www.blogger.com/profile/12905137222286141548noreply@blogger.com