Sunday, 5 July 2015
Monday, 29 June 2015
Sunday, 28 June 2015
Inhibition of the endosymbiont "Candidatus Midichloria mitochondrii" during 16S rRNA gene profiling reveals potential pathogens in Ixodes ticks from Australia.
' However, bacteria of medical significance were detected in I. holocyclus ticks, including a Borrelia relapsing fever group sp., Bartonella henselae, novel "Candidatus Neoehrlichia" spp., Clostridium histolyticum, Rickettsia spp., and Leptospira inadai.'
'Professor Peter Irwin and his colleagues have released the findings from research at Murdoch University. The results have huge implications for the requirement and potential of future research in Australia. Whilst only one tick species (I Holocyclus - aka Paralysis tick) was examined in this study - Borrelia of a relapsing fever species (unidentified) not before found in Australia was discovered. As was numerous other pathogens (Bartonella henselae, novel “Candidatus Neoehrlichia” spp., Clostridium histolyticum, Rickettsia spp., and Leptospira inadai).
What does this mean for Australian Lyme Borreliosis & Co Patients?? In short – It is BIG – and it speaks volumes to the requirements for further urgent research looking at the 70or so other species of ticks in Australia, and the infections they carry. With thousands suffering – Lets hope the Government is listening and provides research funds – and advances plans to put into place better testing and treatment for those chronically ill'
Tuesday, 9 June 2015
Just some of the points raised -
Acquisition of Borrelia Miyamotoi from unfed larval ticks is possible because of transovarial transmission of the pathogen from an infected female.
Human to human transmission by blood transfusion is theoretically possible
A rash was present in only 8% and none described as Erythema Migrans
The diagnosis of Borrelia Miyamotoi in this case series was based on PCR testing and subsequent sequencing.
To date no Borrelia Miyamotoi tests have been approved by US FDA.
A Wright or Giemsa-stained blood smear is a routinely performed procedure which might reveal Borrelia Miyamotoi spirochetes in the blood during febrile episodes.
This emerging research has significance for many countries because Borrelia Miyamotoi has been found in a number of countries including England
Wednesday, 3 June 2015
Identification of new compounds with high activity against stationary phase Borrelia burgdorferi from the NCI compound collection
Jie Feng, Wanliang Shi, Shuo Zhang and Ying Zhang
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
Correspondence: Y Zhang, E-mail: firstname.lastname@example.org
Received 20 March 2015; Revised 22 April 2015; Accepted 8 May 2015
Lyme disease is the leading tick-borne disease in the USA. Whereas the majority of Lyme disease patients with early disease can be cured with standard treatment, some patients suffer from chronic fatigue and joint and muscular pain despite treatment, a syndrome called posttreatment Lyme disease syndrome. Although the cause is unclear, ineffective killing ofBorrelia burgdorferi persisters by current Lyme disease antibiotics is one possible explanation. We took advantage of our recently developed high-throughput viability assay and screened the National Cancer Institute compound library collection consisting of 2526 compounds against stationary phase B. burgdorferi. We identified the top 30 new active hits, including the top six anthracycline antibiotics daunomycin 3-oxime, dimethyldaunomycin, daunomycin, NSC299187, NSC363998 and nogalamycin, along with other compounds, including prodigiosin, mitomycin, nanaomycin and dactinomycin, as having excellent activity against B. burgdorferi stationary phase culture. The anthracycline or anthraquinone compounds, which are known to have both anti-cancer and antibacterial activities, also had high activity against growing B. burgdorferi with low minimum inhibitory concentration. Future studies on the structure–activity relationship and mechanisms of action of anthracyclines/anthraquinones are warranted. In addition, drug combination studies with the anthracycline class of compounds and the current Lyme antibiotics to eradicate B. burgdorferi persisters in vitro and in animal models are needed to determine if they improve the treatment of Lyme disease.
'Of the 2526 compounds in the NCI compound library collection tested, 237 were found to have higher activity against B. burgdorferi persisters than doxycycline and amoxicillin in the primary screen.'
'In summary, we identified the anthracycline class of compounds including daunomycin, daunomycin 3-oxime, dimethyldaunomycin, NSC299187, NSC363998, and nogalamycin along with some other compounds, including prodigiosin, mitomycin, nanaomycin, and dactinomycin, as having excellent activity against both the non-growing stationary phase and growing B. burgdorferi cultures. The structure–activity relationship and mechanisms of action of the anthracycline/anthraquinone class of compounds against B. burgdorferi persisters should be addressed in future studies. In addition, drug combination studies with the anthracycline class of compounds and the current Lyme antibiotics are required to assess whether they improve treatment of Lyme disease in animal models and in patients.'
This latest study follows on from earlier studies posted about
Earlier posts on work by Prof Zhang
Prof Ying Zhang is due to present at this year's LDA conference
Other interesting research on treating Borrelia persister cells by Prof Kim Lewis
Friday, 29 May 2015
Borrelia burgdorferi, the causative agent of Lyme disease, forms drug-tolerant persister cells.Borrelia burgdorferi Persistence: Consensus and Controversy – where do we go from here? This was presented in CDC Webinar on persistence see link above.
Researchers’ discovery may explain difficulty in treating Lyme disease -
This is the first time, we think, that pulse-dosing has been published as a method for eradicating the population of a pathogen with antibiotics that don’t kill dormant cells,” Lewis said. “The trick to doing this is to allow the dormant cells to wake up.”
Other videos of Prof Lewis Principles of Antibiotic Discovery - Kim Lewis
What lights my fire https://www.youtube.com/watch?v=GBZ9hyrMsoM
Prof Lewis featured in a recent BBC documentary on Panorama on his research into finding new antibiotics
and earlier http://www.bbc.co.uk/news/health-30657486